Medicenna Announces Peer-Reviewed Publication of Preclinical Data on MDNA11 in the Journal for ImmunoTherapy of Cancer
-- MDNA11 demonstrates a 30-fold increase in affinity for IL-2 receptor beta without binding to IL-2 receptor alpha when compared to recombinant human IL-2
-- MDNA11 was well-tolerated and induced durable proliferation and expansion of anti-cancer immune cells with limited stimulation of pro-tumor Treg cells in non-human primates
-- MDNA11 alone or in combination with a checkpoint inhibitor generated durable complete responses and provided long-term protection against tumor re-challenge in murine cancer models
“We believe that this prestigious peer-reviewed publication provides important external validation for MDNA11’s preclinical dataset, which demonstrates the advantages of the Superkine’s differentiated ‘beta-only’ approach to targeting the IL-2 receptor,” said
Data presented in the paper are from in vitro, murine, and non-human primate (NHP) studies evaluating MDNA11’s anti-cancer activity as well as its selective IL-2 receptor (IL-2R) binding, pharmacokinetic, pharmacodynamic, and safety profiles. While recombinant human IL-2 (rhIL-2) has been approved for the treatment of metastatic melanoma and kidney cancer, its use is limited by its short half-life and superior binding to the trimeric high-affinity receptor comprising the IL-2R alpha (IL-2Rα), which leads to preferential activation of pro-tumor Treg cells and toxicity. MDNA11 was engineered to overcome these shortcomings by virtue of its vastly superior selectivity and activation of cancer fighting immune cells, via IL-2R beta (IL-2Rβ), unlike “not-alpha” IL-2 variants, which to-date have shown sub-optimal single-agent activity.
Key data and conclusions from the paper include:
In vitro studies:
- MDNA11 demonstrated a 30-fold increase in binding affinity for IL-2Rβ compared to rhIL-2 (MDNA11 KD = 6.6 ± 0.1 nM; rhIL-2 KD = 210 ± 30 nM).
- MDNA11 showed no affinity for IL-2Rα at concentrations up to 2,000 nM MDNA11.
- MDNA11 showed enhanced signaling in anti-cancer T and NK cells and reduced activation of pro-tumor Treg cells when compared to rhIL-2 as shown by 231-fold and 124-fold enhancements in CD8+/Treg and NK/Treg pSTAT EC50 ratios, respectively.
- The terminal half-life of MDNA11 in mice was 25 times greater than that of rhIL-2.
- Cell depletion studies showed that both, CD8+ T cells and NK cells are important for MDNA11 mediated anti-tumor efficacy.
- There was enhanced activation of CD8+ T cells within the tumors as demonstrated by significant increase in expression of intracellular interferon γ.
- MDNA11 alone or in combination with checkpoint inhibitors generated durable complete responses and provided long-term protection against tumor re-challenge in murine cancer models.
- MDNA11 preferentially induced durable proliferation and expansion of anti-cancer immune effector cells (CD8+ T-cells, NK cells and non-Treg CD4+ T-cells), with limited stimulation of pro-tumor Treg cells.
- Proliferation of anti-cancer immune effector cells remained elevated for at least 7 days following treatment with MDNA11.
- MDNA11 was well tolerated. The main safety observations of reduced activity and diarrhea were primarily observed at the highest dose level following the first dose and were generally transient in nature.
In summary, MDNA11’s durable and potent memory response as a single agent together with a favorable safety profile positions the Superkine as a potential long-acting next generation IL-2 for cancer immunotherapy. Being independent of chemical conjugation, pegylation, or complex stearic hindrance techniques to modulate IL-2 activity, MDNA11 provides a versatile framework for a wide range of applications including arming CAR-based cellular therapies, oncolytic viruses, fusion to therapeutic antibodies or development of Bifunctional SuperKine ImmunoTherapies (BiSKITs™) to simultaneously target multiple cytokine pathways.
MDNA11 is currently being evaluated in patients with advanced, relapsed or refractory solid tumors in the Phase 1/2 ABILITY (A Beta-only IL-2 ImmunoTherapY) study. Medicenna expects to provide an update on safety, pharmacokinetic, and pharmacodynamic data from the ABILITY study in the first quarter of 2022. An initial efficacy data update from the study is expected in mid-2022.
About the Phase 1/2 ABILITY Study
The ABILITY (A Beta-only IL-2 ImmunoTherapY) study is designed to assess the safety, pharmacokinetics, pharmacodynamics, and anti-tumor activity of various doses of intravenously administered MDNA11 in patients with advanced, relapsed, or refractory solid tumors. The trial includes an MDNA11 monotherapy arm, as well as a combination arm designed to evaluate MDNA11 with a checkpoint inhibitor. Approximately 80 patients are expected to be enrolled into the ABILITY study. Following establishment of the recommended Phase 2 dose (RP2D) and optimal treatment schedule in the study’s dose escalation phase, Medicenna plans to conduct a dose expansion phase that will enroll patients with renal cell carcinoma, melanoma, and other solid tumors in monotherapy and combination settings. For more information on the ABILITY study, see ClinicalTrials.gov Identifier: NCT05086692.
Medicenna is a clinical stage immunotherapy company focused on the development of novel, highly selective versions of IL-2, IL-4 and IL-13 Superkines and first in class Empowered Superkines. Medicenna's long-acting IL-2 Superkine, MDNA11, is a next-generation IL-2 with superior CD122 (IL-2 receptor beta) binding without CD25 (IL-2 receptor alpha) affinity thereby preferentially stimulating cancer killing effector T cells and NK cells. Medicenna’s early-stage BiSKITs™ program, (Bifunctional SuperKine ImmunoTherapies) is designed to enhance the ability of Superkines to treat immunologically “cold” tumors. Medicenna's IL-4 Empowered Superkine, MDNA55, has been studied in 5 clinical trials including a Phase 2b trial for recurrent GBM, the most common and uniformly fatal form of brain cancer. MDNA55 has obtained Fast-Track and Orphan Drug status from the FDA and FDA/EMA, respectively.
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Source: Medicenna Therapeutics Corp.